Genetic variation in the narrow-clawed crayfish (Astacus leptodactylus) populations as assessed by PCR-RFLP of mitochondrial COI gene

Authors

  • Majidreza Khoshkholgh Department of Fisheries, Faculty of Natural Resources, University of Guilan, Sowmehsara, Iran
  • Sajad Nazari Genetic and Breeding Research Center for Coldwater Fishes, Yasouj, Iran
Abstract:

The genetic variation and population structure of narrow-clawed crayfish (Astacus leptodactylus) was examined by means of polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP) analysis of the cytochrome oxidase subunit I (COI) of mitochondrial DNA. A total of 194 adult specimens were collected from seven sample sites including, two in the south Caspian Sea and one each in Anzali wetland and Aras reservoir and three rivers Chafrood, Masule Rudkhan and Siah Darvishan. The PCR products were digested with 19 restriction enzymes and five enzymes revealed polymorphism patterns (DdeІ, MboІ, TaqI, RsaІ and HinfІ). Twenty eight composite haplotypes were showed with the number of haplotypes in each population sample ranging from 8 to 13. Private haplotypes were found at very low frequencies. Two regional (Siah Darvishan River and Astara) groups were clearly recognized by cluster and molecular variance model (AMOVA) analyses (P

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Journal title

volume 4  issue 4

pages  225- 237

publication date 2015-12-01

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