Genetic variation in the narrow-clawed crayfish (Astacus leptodactylus) populations as assessed by PCR-RFLP of mitochondrial COI gene
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Abstract:
The genetic variation and population structure of narrow-clawed crayfish (Astacus leptodactylus) was examined by means of polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP) analysis of the cytochrome oxidase subunit I (COI) of mitochondrial DNA. A total of 194 adult specimens were collected from seven sample sites including, two in the south Caspian Sea and one each in Anzali wetland and Aras reservoir and three rivers Chafrood, Masule Rudkhan and Siah Darvishan. The PCR products were digested with 19 restriction enzymes and five enzymes revealed polymorphism patterns (DdeІ, MboІ, TaqI, RsaІ and HinfІ). Twenty eight composite haplotypes were showed with the number of haplotypes in each population sample ranging from 8 to 13. Private haplotypes were found at very low frequencies. Two regional (Siah Darvishan River and Astara) groups were clearly recognized by cluster and molecular variance model (AMOVA) analyses (P
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genetic variation in the narrow-clawed crayfish (astacus leptodactylus) populations as assessed by pcr-rflp of mitochondrial coi gene
the genetic variation and population structure of narrow-clawed crayfish (astacus leptodactylus) was examined by means of polymerase chain reaction (pcr) restriction fragment length polymorphism (rflp) analysis of the cytochrome oxidase subunit i (coi) of mitochondrial dna. a total of 194 adult specimens were collected from seven sample sites including, two in the south caspian sea and one each...
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full textEvaluation of different dietary protein and energy levels on growth performance and body composition of narrow clawed crayfish (Astacus leptodactylus)
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Growth, Molting and Survival Response of Juvenile Narrow Clawed Crayfish, Astacus leptodactylus, Fed Two Sources of Dietary Oils
The influence of five pure diets of isonitrogenous and isoenergic with different ratios of Clupeidae fish oil (F) and soybean oil (S) on growth, molting and survival of narrow-clawed crayfish, Astacus leptodactylus, was evaluated during an 8-weeks trial. As soybean oil replaced with fish oil in each treatment, gently. Test diets were inclusive F0/S100, F30/S70, F50/S50, F70/S30 and F100/S0 fis...
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Journal title
volume 4 issue 4
pages 225- 237
publication date 2015-12-01
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